Effect of Glutathione on Oxidant-induced Neurotoxicity in Cultured Spinal Motor Neurons.

Kim,  Tai Yo; Lee,  Jae Sang; Lee,  Kang Chang; Park,  Seung Tak

doi:1997.32.5.732

Experimental Research Article

Korean Journal of Anesthesiology 1997;32(5):732-737.
DOI: https://doi.org/10.4097/kjae.1997.32.5.732

Effect of Glutathione on Oxidant-induced Neurotoxicity in Cultured Spinal Motor Neurons.

Tai Yo Kim, Jae Sang Lee, Kang Chang Lee, Seung Tak Park

¹Department of Anesthesiology, School of Medicine Wonkwang University, Iksan, Korea.
²Department of Anatomy, School of Medicine Wonkwang University, Iksan, Korea.
³Department of Anesthesiology, College of Medicine, Seoul National University, Seoul, Korea.

Abstract

BACKGROUND
Free radical reactions are a part of normal human metabolism. When produced in excess, radicals can cause tissue injury. The present study was aimed to investigate neurotoxic effect of oxygen free radicals and neuroprotective effect of antioxidant(glutathione).
METHODS
Neurotixic effect of oxygen radicals was evaluated by MTT[3-(4,5-dimethylthiazol-2,5- diphenyltetrazolium bromide] assay and neurofilament enzyme-immunoassay after culturing of spinal motor neuron cell line of mouse(NSC-34). Then these cells were exposed to various concentrations of xanthine oxidase(XO) and hypoxanthine(HX). In addition, neuroprotective effect of antioxidant against oxidant-induced neurototoxicity on these cultures was examined.
RESULTS
Exposure of neurons to 25mU/ml XO and 0.2mM HX for 3 hours resulted in a significant cell death and also glutathione(GSH) blocked the neurotoxicity induced by oxygen radicals in cultured mouse spinal motor neurons.
CONCLUSION
From the above results, it is suggested that oxygen radicals are toxic in NSC-34. Selective antioxidants such as GSH are effective in blocking oxidant-induced neurotoxicity on these cultures.

Key Words: Cells; cultured; motor neuron cells; Oxygen; oxygen free radicals; Toxicity; neurotoxicity