Korean J Anesthesiol Search

CLOSE


Korean Journal of Anesthesiology 2009;57(3):331-336.
DOI: https://doi.org/10.4097/kjae.2009.57.3.331   
Effects of propofol and etomidate on hydrogen peroxide-induced oxidative damage in hepatocyte.
Eun Ho Lee, Jin Woo Shin, Sun kyung Yoon, Hyo jung Son, Ji Yeon Lee, Seung Woo Ku, Joung Uk Kim, Yu Mi Lee
1Department of Anesthesiology and Pain Medicine, Asan Medical Center, University of Ulsan School of Medicine, Korea. swkoo@amc.seoul.kr
2Korea Institute of Radiologic and Medical Science, Seoul, Korea.
Abstract
BACKGROUND
The present investigation was undertaken to evaluate the protective effect of propofol and etomidate against hydrogen peroxide (H2O2) induced oxidative damage in human hepatic SNU761 cells by measuring lactate dehydrogenase (LDH). METHODS: The cell line of human hepatocellular carcinoma was grown for 24 hours in dissociated cell culture. They were divided into eight groups: negative control (NC) group with no drug administration, positive control (PC) group with H2O2 250 micrometer and other groups pretreated with propofol (P; 1, 10, 50 micrometer) or etomidate (ET; 1, 10, 50 micrometer) followed H2O2 administration. After 7 hours, cell death was assessed by morphology under the light microscope and quantified by measuring the LDH in the culture media.
RESULTS
In the light microscopic findings, the intact cells were increased in all three propofol groups compared to group PC. H2O2-induced LDH production was also significantly suppressed in all three propofol groups compared to group PC (P < 0.001). There were no significant differences in the microscopic findings and LDH production between the etomidate groups and group PC. CONCLUSIONS: These results suggest that the propofol has protective effect on the hepatocyte against H2O2-induced oxidative stress.
Key Words: Etomidate; Hydrogen peroxide; Lactate dehydrogenase; Oxidative stress; Propofol


ABOUT
ARTICLE CATEGORY

Browse all articles >

BROWSE ARTICLES
AUTHOR INFORMATION
Editorial Office
101-3503, Lotte Castle President, 109 Mapo-daero, Mapo-gu, Seoul 04146, Korea
(서울특별시 마포구 마포대로 109 롯데캐슬 프레지던트 101동 3503호)
Tel: +82-2-792-5128    Fax: +82-2-792-4089    E-mail: journal@anesthesia.or.kr                
Business Name: Korean Society of Anesthesiologists (대한마취통증의학회)
Business Registration: 106-82-07194
Representative: Jun Heum Yon (연준흠)

Copyright © 2024 by Korean Society of Anesthesiologists.

Developed in M2PI

Close layer
prev next