| Effect of Etomidate on N-methyl-D-aspartate Induced Neurotoxicity in Rat Cortical Neurons. |
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Yu Mi Lee, Eun Ho Lee, Joung Uk Kim, Hyun Jung Lee, Byeong Moon Hwang, Jin Woo Shin, Seung Woo Ku |
1Department of Anesthesiology and Pain Medicine, College of Medicine, University of Ulsan, Seoul, Korea. jukim@amc.seoul.kr
2Department of Anesthesiology and Pain Medicine, College of Medicine, University of Kangwon, Chuncheon, Korea.
3Asan Institute of Life Science, Seoul, Korea. |
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| Abstract |
BACKGROUND
The present investigation was undertaken to evaluate the neuroprotective effect of etomidate against N-methyl-D- aspartate (NMDA) induced neurotoxicity in rat cortical neurons by measuring lactate dehydrogenase (LDH).
METHODS
The cerebral cortical neurons of fetal rat were grown for 12 days in dissociated cell culture. They were divided into four groups: first group acted as control with no drug administration and other groups treated with either NMDA 100microM or Etomidate (ET) 10microM + NMDA 100microM or ET 100microM + NMDA 100microM After 24 hrs, cell death was assessed by morphology under the light microscope and quantified by measuring the LDH in the culture media.
RESULTS
In the light microscopic findings, the intact cortical neurons were increased in the ET groups.
NMDA-induced LDH production also significantly suppressed in ET group (P<0.05). There were no significant difference between the ET 10microM and 100microM groups.
CONCLUSIONS
These results suggest that the etomidate has protective effect on the cultured rat cortical neurons against NMDA-induced neurotoxicity. |
| Key Words:
etomidate; lactate dehydrogenase (LDH); neurotoxicity; N-methyl-D-aspartate (NMDA) |
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