| Genotoxicity of Enflurane in Human Peripheral Blood Lymphocytes Studied in vivo by Single Cell Gel Electrophoresis. |
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Se Hyun Lew, Dong Won Kim, Jung Kook Suh, Ik Sang Seung, Jae Chul Shim, Mi Ae Cheong, Jang Hwan Park |
1Department of Anesthesiology and Pain Medicine, Hanyang University College of Medicine, Seoul, Korea. dongwkim@hanyang.ac.kr
2Department of Microbiology, Hanyang University College of Medicine, Seoul, Korea. |
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| Abstract |
BACKGROUND
The alkaline single cell gel electrophoresis comet assay was applied to study the genotoxic properties of enflurane on the human peripheral blood lymphocytes (PBL) of cancer patients before and during anesthesia as compared to an non-cancer control group.
Method
The cancer group consisted of 24 patients (aged 15-77 years), while the control group consisted of 14 trauma individuals (aged 20-81 years). After anesthesia induction (thiopental 4 mg/kg and vecuronium 0.1 mg/kg), it was maintained by enflurane inhalation; 1-2 minimal alveolar concentration in oxygen - nitrous oxide mixture. Venous blood samples were obtained before the induction of anesthesia, and after 60 and 120 min of anesthesia. The comet assay detects DNA damage, such as strand breaks and alkaline labile sites induced directly by genotoxic agents, and DNA degradation due to cell death. Fifty cells from each sample were examined and Olive tail moments (OTM) were calculated using Komet 4TM software.
RESULTS
OTM values were no different between controls and patients before anesthesia. However, the OTMs of blood sampled from cancer patients at 60 (7.97 +/- 1.83) and 120 min (7.86 +/- 2.05), and from trauma patients at 120 min (8.04 +/- 1.32) of anesthesia were significantly increased.
CONCLUSIONS
In immunocompromised cancer patients, we suggest the existence of a higher risk of an association DNA damage and enflurane exposure. |
| Key Words:
DNA; enflurane; lymphocyte; single cell gel electrophoresis |
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