| The Role of the Glutamate Receptor in the Transient Focal Cerebral Ischemia in Rats. |
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Chang Hee Chae, Hae Kyu Kim, Kyung Hoon Kim, Seong Wan Baik, Inn Se Kim |
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Department of Anesthesiology, College of Medicine, Pusan National University, Pusan, Korea. |
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| Abstract |
BACKGROUND
To investigate the role of glutamate in the transient focal cerebral ischemia, a reversible middle cerebral artery occlusion model was induced in 25 male Sprague-Dawley rats.
METHODS
Triphenyltetrazolium chloride (TTC) stain was used for evaluation of the changes of infarction ratio in MK-801 (0.3, 1.0, 3.0 mg/kg) or pentylenetetrazole (50 mg/kg) treated groups.
RESULT
The infarction ratio at 48 hours after 2 hour transient focal brain ischemia was 39.2 +/- 13.2% in control group and 23.8 +/- 4.2, 27.0 +/- 8.9, and 12.8 +/- 4.4% in MK-801 (0.3, 1.0, 3.0 mg/kg) groups. In the pentylenetetrazole (PTZ) group, the infarction ratio was 32.6 +/- 6.7%.
CONCLUSIONS
The non-specific glutamate receptor antagonist, MK-801, showed a trend toward dose-dependent improvement, but the PTZ group showed no improvement. From these results, it suggested that glutamate might be partly involved in the mechanisms of ischemia-induced neuronal damage. |
| Key Words:
Animal: rats; Brain: edema; middle cerebral artery occlusion; Pharmacology: penty lenetetrazole; MK-801 |
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